| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Lack of evidence for Litopenaeus vannamei Toll receptor (lToll) involvement in activation of sequence-independent antiviral immunity in shrimp
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| Autores: |
Labreuche, Yannick
O'Leary, Nuala A.
De La Vega, Enrique
Veloso, Artur
Gross, Paul S.
Chapman, Robert W.
Browdy, Craig L.
Warr, Gregory W.
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| Data: |
2009-07
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| Ano: |
2009
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| Palavras-chave: |
Shrimp
Virus
RNA interference
Invertebrate immunity
Innate immune response
Toll like receptor
DSRNA
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| Resumo: |
injection of non-specific dsRNA initiates a broad-spectrum innate antiviral immune response in the Pacific white shrimp, Litopenaeus vannamei, however, the receptor involved in recognition of this by-product of viral infections remains unknown. In vertebrates, dsRNA sensing is mediated by a class of Toll-like receptors (TLRs) and results in activation of the interferon system. Because a TLR (lToll) was recently characterized in L. vannamei, we investigated its potential role in dsRNA recognition. We showed that injection of non-specific RNA duplexes did not modify lToll gene expression. A reverse genetic approach was therefore implemented to study its role in vivo. Silencing of lToll did not impair the ability of nonspecific dsRNA to trigger protection from white spot syndrome virus and did not increase the shrimp susceptibility to viral infection, when compared to controls. In contrast, gene-specific dsRNA injected to specifically silence lToll expression activated an antiviral response. These data strongly suggest that shrimp lToll plays no role in dsRNA-induced antiviral immunity. (C) 2009 Elsevier Ltd. All rights reserved.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/2009/publication-6428.pdf
DOI:10.1016/j.dci.2009.02.005
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| Editor: |
Elsevier
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| Relação: |
http://archimer.ifremer.fr/doc/00000/6428/
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| Formato: |
application/pdf
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| Fonte: |
Developmental & Comparative Immunology (0145-305X) (Elsevier), 2009-07 , Vol. 33 , N. 7 , P. 806-810
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| Direitos: |
2009 Elsevier Ltd All rights reserved.
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